Colorimetric Determination of Nicotinamide with Dichlorodicyano Benzoquinone

Abstract

A Simple and Spectrophotometric method has been developed for the determination of nicotinamide with 2, 3- dichloro -5, 6-dicyano-1, 4- benzoquinone (DDQ). The method is based on charge transfer reaction between nicotinamide and DDQ. The Stoichiometric relationship between nicotinamide and DDQ shows a 2:1 mole ratio. A shift in absorption maxima was noted at 468nm. Beers law was evident between the concentrations of 10μg/ml - 90 g/ml with molar absorptivity of 0.37 x103 L mol-1cm-1and Sandell’s sensitivity of 1.0 per 0.001absorbance unit. The proposed method has been successfully applied in the determination of nicotinamide in pure and commercial forms.