Please use this identifier to cite or link to this item: http://repository.unizik.edu.ng/handle/123456789/312
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dc.contributor.authorEsimone, Charles Okechukwu-
dc.contributor.authorOmeje, Edwin Ogechukwu-
dc.contributor.authorOsadebe, Patience Ogoamaka-
dc.contributor.authorNworu, Chukwuemeka Sylvester-
dc.contributor.authorNwodo, Justina Ngozi-
dc.contributor.authorKawamura, Akira-
dc.contributor.authorProksch, Peter-
dc.date.accessioned2022-11-18T17:09:39Z-
dc.date.available2022-11-18T17:09:39Z-
dc.date.issued2011-10-12-
dc.identifier.citationPharmaceutical Biology, 49:12, 1271-1276,en_US
dc.identifier.urihttps://doi.org/10.3109/13880209.2011.621129-
dc.identifier.urihttp://repository.unizik.edu.ng/handle/123456789/312-
dc.description.abstractContext: The Eastern Nigeria mistletoe, Loranthus micranthus Linn. (Loranthaceae), is used in the treatment of severaldiseases including immune-modifying diseases and thus there is a need to identify the immunoactive constituents. Objective: This research isolated and characterized the immunoactive constituents in the Eastern Nigeria mistletoe. Materials and methods: Bioassay-guided fractionation was employed in the isolation and purification of the constituents. The characterized compounds were screened for immunostimulatory activities on isolated C57BL/6 mice splenocytes and early activation marker, CD69 at concentrations of 10, 25, and 100 µg/mL using flow cytometry techniques and compared to lipopolysaccharide (LPS; 10 µg/mL) and concanavalin A (ConA; 2 µg/mL) as standards. Results: Two compounds, a novel sesquiterpene, 2, 3-dimethoxy-benzo [a, b] cyclopentenyl-3′,3′,5′-trimethyl pyran4-carboxylic acid (1), and a known alkaloid, lupinine (2) were isolated and characterized. The compounds (25 µg/ mL) showed statistically significantly (p<0.05) stimulatory activity on the splenocytes with values of 56.34±0.26% and 69.84±0.19%, respectively, compared to 7.58±0.42% recorded for the unstimulated control. Similarly, the CD69 expression assay showed immunostimulation with statistically significant values (p<0.05) of 2.31±0.07% and 2.71±0.03%, respectively, compared to 1.69±0.05% recorded for the non stimulated control. Discussion: These data suggest that the isolated compounds possess immunomodifying abilities. In addition, the activation of the CD69 molecule is possibly one of its mechanisms of action. Conclusion: These compounds may be responsible in part, for the immunostimulatory activities already established for the Eastern Nigeria mistletoes.en_US
dc.language.isoenen_US
dc.publisherPharmaceutical Biologyen_US
dc.subjectSesquiterpenoiden_US
dc.subjectimmunomodulationen_US
dc.subjectC57BL6 splenocytesen_US
dc.subjectnatural producten_US
dc.subjectcell proliferationen_US
dc.subjectchromatographyen_US
dc.subjectmedicinal plantsen_US
dc.titleA novel sesquiterpene acid and an alkaloid from leaves of the Eastern Nigeria mistletoe, Loranthus micranthus with potent immunostimulatory activity on C57BL6 mice splenocytes and CD69 moleculeen_US
dc.typeArticleen_US
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